NEB Tools brings New England Biolabs’ most popular web tools to your iPhone. Use Enzyme Finder to select a restriction enzyme by category or recognition sequence, or search by name to find information on any NEB enzyme. Sort your results so they make sense to you, then email them to your inbox or connect directly to www.neb.com.
To help select the best conditions for double digests, this chart shows the optimal (supplied) NEBuffer and approximate activity in the four standard NEBuffers and NEBuffer EcoRI for each enzyme. In addition, the table also shows recommended reaction temperature, heat inactivation temperature, recommended diluent buffer and whether the enzyme is Time-Saver qualified (i.e., cleaves substrate in 5 minutes).
Cleaving a DNA substrate with two restriction enzymes simultaneously (double digestion) is a common timesaving procedure. Use this tool to select reaction conditions amenable to any two NEB restriction enzymes.
Use this tool to select restriction enzymes by name, sequence, overhang, or type.
The Restriction Enzyme Database.
This list contains isoschizomers (Restriction endonucleases that recognize the same sequence) for commercially available restriction endonucleases. It also specifies which isoschizomer is available from New England Biolabs.
There are already enough variables in PCR, don't let polymerase performance be one of them. Choose from one of the largest selections of polymerases for PCR applications from the leader in enzyme technology and bring unparalleled confidence to your experiments.
The nucleotide sequence files available are those used to the produce the plasmid vector, viral and bacteriophage maps contained in New England Biolabs Catalog as well as the tables containing the locations of sites.
This tool will take a DNA sequence and find the large, non-overlapping open reading frames using the E.coli genetic code and the sites for all Type II and commercially available Type III restriction enzymes that cut the sequence just once. It also shows the enzymes which could be used in a complete digest to excise each open reading frame that it finds. From the initial display there are options to go further, including custom digestion with enzymes of your choice and various displays of the digest.